Screening for cephalosporin-resistant Streptococcus pneumoniae with the Kirby-Bauer disk susceptibility test
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منابع مشابه
Screening for cephalosporin-resistant Streptococcus pneumoniae with the Kirby-Bauer disk susceptibility test.
Kirby-Bauer disk susceptibility tests with five standard cephalosporin disks were performed on 23 penicillin-resistant Streptococcus pneumoniae isolates for which ceftriaxone MICs were 0.125 to 4 micrograms/ml. Cefuroxime disk inhibition zone diameters distinguished clearly isolates for which ceftriaxone MICs were > or = 2 micrograms/ml from more susceptible strains, whereas cephalothin, ceftiz...
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Standard Bauer-Kirby disk tests were performed with 85 selected isolates, each tested in triplicate by four different investigators. Each disk test was observed, and zone diameters were measured, under two lighting conditions (transmitted light and reflected light). The two lighting systems produced similar zone measurements (+/-2 mm) with 96% of the tests. When there were greater differences, ...
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susceptibility test were performed on Mueller-Hinton agar with 5% horse blood according to NCCLS recommendations with the following discs: cefuroxime (30/jg), ceftizoxime (30 fig), cefpodoxime (30 /ig), cefixime (30 fig), cefaclor (30 ug) and loracarbef (10 and 30/ig). Agar and discs were from Sanofi Diagnostic Pasteur (Marnes-la-Coquette, France) except loracarbef prepared from standard refere...
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A rapid (6-7 hr), modified Kirby-Bauer disk-susceptibility method, by which derivatives of tetrazolium dyes are used to enhance delineation between areas of growth and zones of inhibition, has been developed. Inoculated petri plates, prepared by the Kirby-Bauer method, were sprayed, after 6 to 7 hr of incubation (37 C), with aqueous solutions of MTT-tetrazolium or INT-tetrazolium resulting in r...
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Antimicrobial susceptibility testing by the disc diffusion method is discussed with emphasis on the need for standardization of methodology. The KirbyBauer method is currently widely used by clinical laboratories and best incor porates many of the features recommended by the World Health Organization. It is relatively simple from a technicological standpoint and accurate and reproducible so lo...
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ژورنال
عنوان ژورنال: Journal of Clinical Microbiology
سال: 1994
ISSN: 0095-1137,1098-660X
DOI: 10.1128/jcm.32.1.274-275.1994